- Cool Tool – Online Cell Culture Media Formulation ToolPosted 3 days ago
- Video – Impact of Chemically Defined Media on Product QualityPosted 3 days ago
- Ask the Expert – Media Optimization Can Improve Glycosylation Patterns and Consistency to Impact Protein EfficacyPosted 5 days ago
- Digital Biomanufacturing Will Enable Tissue BioprintingPosted 2 weeks ago
- Video – When and Where to Optimize Cell Culture MediaPosted 2 weeks ago
- Cool Tool – SCOUT® technology reduces time to market and increases chance of success for biopharmaceutical productsPosted 3 weeks ago
- Pumping Iron – But Not in the gym: The Critical Roles of Transferrin in Cell Culture MediaPosted 3 weeks ago
- Solve Production Challenges of Difficult to Express Proteins with Scalable, Continuous ManufacturingPosted 3 weeks ago
- Video Tutorial – Understanding Chemically Defined Media in BioprocessingPosted 4 weeks ago
- 2016 BioProcess International Award Winners – Upstream and AnalyticalPosted 4 weeks ago
Using the Corning HYPERStack Cell Culture Vessel and the Enhanced Attachment Microcarriers for Scale-Up and Production in the Vaccine Industry
Katherine E. Strathearn, Ph.D., Mansi Soni, and Mark E. Rothenberg, Ph.D.
Viral transductions are used at the preclinical and clinical stages in the vaccine industry leading to an increase in demand to produce more virus more efficiently. The Corning® HYPER technology and microcarrier beads offer the ability to increase efficiency by increasing surface area without increasing spatial footprint. The focus of this study was (i) to determine the efficacy of generating Adeno- and lentiviral particles using the unique Corning HYPER technology, and (ii) to evaluate the use of the Enhanced Attachment microcarrier beads on cell lines typically used in the vaccine-producing industry (e.g. Vero, and HEK-293).
To assess viral production on the HYPER technology HEK-293AD (adenovirus) or HEK-293LTV (lentivirus) were transduced (adeno) or transfected (lenti) on the HYPERStack-12 to produce the viral particles. The results demonstrated that adeno- and lenti-viral particles can be generated in the Corning HYPERStack® vessel at similar titers compared to traditional tissue culture vessels, while allowing for greater virus production in a smaller footprint.
To optimize cell scale-up on the Corning microcarrier beads various conditions were evaluated using multiple vaccine producing cell types. The data presented here demonstrate Vero and HEK-293AD cell expansion on Corning Enhanced Attachment microcarriers.