Semi-solid cloning of mouse hybridomas to generate mouse monoclonal antibodies

By on March 17, 2014

This video outlines the steps in semi-solid cloning of mouse hybridomas to generate mouse monoclonal antibodies using ClonaCell™-HY. The cloning protocol demonstrated in this video can also be used to clone other mammalian cell types with other ClonaCell™ semi-solid media products. Steps covered in this video include preparation of myeloma cells and splenocytes, fusion, selection and cloning, and harvesting

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