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Transfection Optimization for Improved Efficiency and Performance
Transfection is a common, yet sophisticated method that is frequently used to artificially deliver nucleic acids (DNA or RNA) into cells for a variety of applications. To efficiently introduce nucleic acids to the cell, a chemical method such as lipid based reagents or a physical method such as electroporation is most commonly used. These nucleic acids can alter properties of the cell, allowing for the study of gene function and protein expression within the context of the cell. However, there are a number of important factors, such as reagent dose, nucleic acid dose, cell density, complexation media, incubation time, etc., that can affect the efficiency of transfection. The difference between a good and bad transfection, can ultimately determine how many times an experiment will be repeated. Understanding the interaction between these key factors and the importance of optimization for a particular cell type can help to reduce the cost and consumption of time and reagents.
Post your questions relating to any of the many steps involved with transfection: general culturing of cells, the N:P ratio of the transfection complex, media changes, etc.
This Ask the Expert Session is Sponsored by Life Technologies and hosted by Nektaria Andronikou. Nektaria joined Life Technologies in 2010 and is currently working with the transfection team on the development of new delivery methods targeting relevant cellular models that will enable the use of exciting new technologies. She received a Bachelor of Science in Biochemistry with a minor in Cellular and Molecular Biology from UCSD. She began her professional career at ISIS Pharmaceuticals, as a research associate for the Cardiovascular Drug Discovery program, screening numerous pre-clinical targets that led to the discovery of the now FDA approved antisense drug, Kynamro.