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Hematopoietic Stem & Progenitor Cell Culture
The culture of hematopoietic stem and progenitor cells (HSPCs) is important to assay the quality and functional properties of HSPCs e.g., during the implementation of transplantation or other approaches to treat hematological disorders, such as leukemia. Examples of HSPC culture assays include colony-forming unit (CFU) assays in semi-solid methylcellulose-based media, such as MethoCult™, to identify and quantify HSPCs, and expansion cultures in liquid media, such as StemSpan™, to increase HSPC numbers or generate large numbers of mature blood cells. These culture methods can also used to evaluate the efficacy and toxicity of new drug candidates on hematopoiesis in vitro and to generate target cells for reprogramming to generate induced pluripotent stem cells.
In this Ask the Expert Session, we will discuss challenges and techniques for the culture of HSPCs, including the use of serum-free, defined media and supplements that have been developed by STEMCELL Technologies for HSPC culture applications.
This session is sponsored by
Dr. Wognum obtained his PhD at the University of Amsterdam in the Netherlands and worked as a postdoctoral fellow at the Terry Fox Laboratory in Vancouver, Canada, and the Department of Hematology at Erasmus University in Rotterdam, the Netherlands. He studied the role of hematopoietic growth factors and their receptors in normal hematopoiesis and leukemia and contributed to preclinical studies aimed at improving hematopoietic recovery after cytoreductive therapy and transplantation. Questions about the culture of of HSPCs?
During this Ask the Expert session, Dr. Bert Wognum will be answering your questions about StemSpan™ media and supplements for serum-free HSPC expansion, along with highlighting recent research regarding the use of small molecules for HSPC expansion.
Topics of discussion may include:
- The best cell sources for HSPCs
- Methods to measure HSPC frequencies and properties in culture
- When to purify HSPCs and how (or when not to) for culture
- How to use HSPC cultures to measure the hematotoxicity of new drug candidates
- How to exploit molecular regulators of HSPC function to drive HSPC expansion in culture
- Methods to generate large numbers of specific blood cells (e.g. erythrocytes and platelets) by promoting HSPC expansion and lineage-specific differentiation.
- The use of HSPCs and culture-expanded erythroid cells as cell sources for reprogramming to generate pluripotent stem cells
- Examples of the successful translation of methods to improve outcomes after clinical HSPC transplantation