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Optimization of serum free media for the propagation of primary and stem cells in animal and human serum component free conditions
Optimization of serum free media can be a complex and daunting challenge for scientists working to bring a cellular therapy to market. When considering raw material sourcing, it is important to limit animal or human serum derived components as these are major sources of variability and potential viral contamination risks. Inclusion of recombinant versions represents a viable alternative to circumvent issues associated with serum-derived proteins. However, utilization of these components can sometimes bring new hurdles to light.
Here, I discuss serum free media design and formulation for an array of primary and stem cell systems. Using recombinant animal free proteins, I can provide guidance to convert any media to be completely void of any human serum or animal serum-derived components. Further, through this guidance, I can share some tricks to the trade that I’ve learned along the way to maximize performance of your cell system.
This Ask the Expert session is sponsored by InVitria and is hosted by Randall Alfano. Mr. Alfano, Cell Culture Scientist, joined InVitria in 2012. He currently develops animal free proteins and supplements for various cell systems including stem cells. He has over 6 years’ experience in recombinant protein expression and purification as well as medium development for CHO-based biomanufacturing and stem cells. Randall was awarded his Ph.D. in 2009 from the Texas A&M College of Medicine Health Science Center in Cell Biology.