Optimization of serum free media for the propagation of primary and stem cells in animal and human serum component free conditions

Sponsored by: Invitria
Session ends: October 30th, 2015, 3:00pm MST
Answers by: Randall Alfano, Cell Culture Scientist, Invitria


Optimization of serum free media can be a complex and daunting challenge for scientists working to bring a cellular therapy to market. When considering raw material sourcing, it is important to limit animal or human serum derived components as these are major sources of variability and potential viral contamination risks. Inclusion of recombinant versions represents a viable alternative to circumvent issues associated with serum-derived proteins. However, utilization of these components can sometimes bring new hurdles to light.

Here, I discuss serum free media design and formulation for an array of primary and stem cell systems. Using recombinant animal free proteins, I can provide guidance to convert any media to be completely void of any human serum or animal serum-derived components. Further, through this guidance, I can share some tricks to the trade that I’ve learned along the way to maximize performance of your cell system.

This Ask the Expert session is sponsored by InVitria and is hosted by Randall Alfano. Mr. Alfano, Cell Culture Scientist, joined InVitria in 2012. He currently develops animal free proteins and supplements for various cell systems including stem cells. He has over 6 years’ experience in recombinant protein expression and purification as well as medium development for CHO-based biomanufacturing and stem cells. Randall was awarded his Ph.D. in 2009 from the Texas A&M College of Medicine Health Science Center in Cell Biology.

please take advantage of the opportunity to ask our expert a question and participate in a lively discussion on designing serum free cell culture media.

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Questions & Answers

Do you have any recommendations on a coating matrix that you use for MSCs?

If you are using serum-containing medium, a tissue culture-treated flask should work just fine without the need for any additional treatment for good MSC growth. However, once you withdraw serum, MSC adhesion can become an issue. 1-5 ug/squared centimeter of human serum fibronectin is probably the simplest way to recover MSC adhesion in the absence […]» Read More

We don’t have an immediate need to eliminate serum in our culture but are seeing some problems with variability. Do you see a reduction in variability issues with serum reduction and how does that compare with eliminating altogether? MSCs

In my experience with serum-based media in expanding MSC, I’ve found that reducing serum does seem to improve variability issues. However, improvement would depend on the extent of serum reduction as well as the composition of the substituted defined formulation. With this said, serum reduction does have some issues. Generally speaking, the problem with serum […]» Read More

I am working on reducing serum in our iPCS culture. What steps would you recommend for weaning and in your experience how low have you gotten in serum concentration?

I would highly recommend a step wise reduction in serum for iPSC over multiple passages. There are a variety of serum free medias currently marketed for iPSCs, so these cells can be propagated in serum free conditions. In addition, we have developed in house animal component free formulations that are capable of expanding iPSC for […]» Read More

In your opinion if you have determined that you can’t get rid of serum completely is there any advantage (beyond cost) to trying to reduce it?

Total elimination of serum versus serum reduction will be determined by the end application of the cell-based product. For therapeutic applications, it is best to totally eliminate serum for variability and safety reasons. If you find that you are having difficulty in removing serum completely, I would be interested in talking with you further to […]» Read More

We would like to initiate a large media component screen to optimize our MSC production. The challenge is that for what we want to screen it is going to be very time/labor intense. Can you advise on your experience using automation to simplify this?

Automation of cell culture processes is a great way to simplify media component screening. However, it is quite pricey. If you have the resources to incorporate automation of media development, there are several options. If there are limiting resources, there are specific methods and techniques that one can use to minimize the cost of media […]» Read More