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Pluripotent stem cell (PSC) culture
Sponsored by: Life Technologies
Session ends: April 25th, 2013, 3:00pm MST
Answers by: Dr. Nirupama (Rupa) Shevde, Customer Training Manager in the Primary and Stem Cell Systems group, Life Technologies
Pluripotent stem cell (PSC) culture has evolved over the past decade, just as stem cell researcher needs have been changing and the market has been growing. Initial culture conditions for stem cells used FBS and mouse embryonic feeders (MEFs). The need for a more defined system brought about the introduction of KnockOut™ Serum Replacement (KSR) in 1997 for the culture of mouse embryonic stem cells (ESCs). Even today, KSR has remained an integral part of the feeder based workflow for both mouse and human PSC culture. The desire to move away from the variability and work required to maintain MEFs led to the development of feeder-free media systems. There are a number of options for feeder-free based media and matrices; StemPro® hESC SFM and Essential 8™ media are two robust options available and both work with Geltrex® and Vitronectin matrices. As choice of media systems for PSCs grows, understanding which media to use for a specific application becomes an important consideration.
Join Dr. Nirupama (Rupa) Shevde, customer training manager at Thermo Fisher Scientific, to ask your questions regarding the culture of pluripotent stem cells. Questions can range from best culture options and technologies for deriving pluripotent stem cells to how to adapt cells to a new media and everything in between.