- Non-Invasive Stem Cell Characterization and Predicting Differentiation using Luminex AssaysPosted 17 hours ago
- CRISPR Technology Where it Stands and What the Future HoldsPosted 6 days ago
- Ask the Expert – Using Luminex Technology to Non-Invasively Monitor Stem Cell DifferentiationPosted 1 week ago
- Controlling Cell Culture pH in BioreactorsPosted 2 weeks ago
- The Impact of Aeration on Cell Culture OptimizationPosted 4 weeks ago
- Cool Tool – Novel universal titer boost and enhancer improves CHO cell protein production in small bioreactorsPosted 1 month ago
- The Dish’s Weekly Biotechnology News Wrap Up – September 16, 2016Posted 1 month ago
- Utilizing UVC LEDs in Biotech and Pharma – Paving the way to better instrument design and better manufacturing methodsPosted 1 month ago
- Synopsis: An Interview with Audrey Jia, former FDA CMC Reviewer for Biological ProductsPosted 1 month ago
- Predicting Differentiation and Characterizing Pluripotent Stem Cells Using Non-invasive Multi-analyte Luminex® AssaysPosted 1 month ago
Pluripotent stem cell (PSC) culture
Sponsored by: Life Technologies
Session ends: April 25th, 2013, 3:00pm MST
Answers by: Dr. Nirupama (Rupa) Shevde, Customer Training Manager in the Primary and Stem Cell Systems group, Life Technologies
Pluripotent stem cell (PSC) culture has evolved over the past decade, just as stem cell researcher needs have been changing and the market has been growing. Initial culture conditions for stem cells used FBS and mouse embryonic feeders (MEFs). The need for a more defined system brought about the introduction of KnockOut™ Serum Replacement (KSR) in 1997 for the culture of mouse embryonic stem cells (ESCs). Even today, KSR has remained an integral part of the feeder based workflow for both mouse and human PSC culture. The desire to move away from the variability and work required to maintain MEFs led to the development of feeder-free media systems. There are a number of options for feeder-free based media and matrices; StemPro® hESC SFM and Essential 8™ media are two robust options available and both work with Geltrex® and Vitronectin matrices. As choice of media systems for PSCs grows, understanding which media to use for a specific application becomes an important consideration.
Join Dr. Nirupama (Rupa) Shevde, customer training manager at Thermo Fisher Scientific, to ask your questions regarding the culture of pluripotent stem cells. Questions can range from best culture options and technologies for deriving pluripotent stem cells to how to adapt cells to a new media and everything in between.