I am looking for a reliable transfection protocol for DU 145 prostate cancer research. Any recommendations?

This question is part of the following Ask The Expert session:

Cancer Research

Answered by:

Paula Flaherty

Company: Corning

Job Title: Technology Manager

Answer

Although Corning does not have any DU145 specific transfection protocols, we do know that DU145 as well as many other prostate lines behave differently in 3D as opposed to 2D. 3D transfection is possible in Corning® Matrigel® matrix, as well as in Corning Ultra Low Attachment vessels and Corning spheroid microplates. There are several protocols for 3D transfection that can be found in the literature as well as some commercially available 3D transfection kits. We’ve included some helpful links below:

Influence of Matrices on 3D-Cultured Prostate Cancer Cells’ Drug Response and Expression of Drug-Action Associated Proteins

Transfection of human prostate cancer cells (DU145) with the human short prolactin receptor sensitizes the cells to cell cycle regulation by S179D prolactin

DU-145 and PC-3 human prostate cancer cell lines express androgen receptor: Implications for the androgen receptor functions and regulation

Gene transfection to spheroid culture system on micropatterned culture plate by polyplex nanomicelle: a novel platform of genetically-modified cell transplantation

Protein Transfection Study Using Multicellular Tumor Spheroids of Human Hepatoma Huh-7 Cells

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