How many rounds of secretion profiles did it take you to start to see a differentiation pattern?

Answer

We are still undergoing this process to find signatures that will correlate with differentiation success.  We have done up to 3 replicates at this point and are focusing on molecules that were seen both in the Array and confirmed via Luminex.  We found very consistent results.  It will be important to then test these analytes across more cell lines as well as different protocols to confirm our findings.

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