Do we need Poloxamer 188 in seed train?

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Company: Merck

Job Title: Head of Perfusion Systems Lab, Life Science, BioProcessing R&D,

Answer

Yes, Poloxamer 188 in seed trains is needed to adapt the cells to the media composition and to protect the cells from shear. Especially after thaw, cells are very sensitive.

This post is sponsored by MilliporeSigma.

Answer

Does the new poloxamer provide any other benefits beyond protection from shear or cause any issues that users should be aware of?

What are minimal criteria when we do NOT need to use Poloxamer (cell concentration, tip speed, bioreactor size, pore sparger size, metabolic activity, etc)

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