Question:

My question is about the future potential for Matrigel. Specifically, I’m interested in how Matrigel might be used as a bioink for 3D bioprinting. Have people been bioprinting with their cells in a Matrigel mixture? If so, what kinds of results have been seen (any publications I could read?) Besides bioprinting, are there any other new 3D cell culture techniques on the horizon?

The Answer:

Corning® Matrigel® is poised to play an integral role in many 3D cell culture techniques, including bio-printing. Scientists have been using it to print many different tissues types. Listed below is a table that summarizes articles that have been published in this space recently that use Corning Matrigel in 3D bioprinting. Other 3D techniques that use Matrigel matrix are microfluidics and organ-on-a-chip for scaffold systems. For scaffold-free systems, Corning provides spheroid plates where the user can generate and analyze 3D spheres formed by one or more cell types. There is often a cross use of spheroid plates with Matrigel matrix if the customer is interested in generating self-assembled 3D structures.

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Literature summary: Use of Corning® Matrigel® Basement Membrane Matrix in bio-printing
Literature Title Application/Cell Types
Prolonged presence of VEGF promotes vascularization in 3D bioprinted scaffolds with defined architecture Vascular
human endothelial progenitor cells (EPCs)
Distinct tissue formation by heterogeneous printing of osteo- and endothelial progenitor cells Bone Tissue Engineering
SCs, endothelial progenitor cells (EPCs)
Bioprinting cell-laden matrigel for radioprotection study of liver by pro-drug conversion in a dual-tissue microfluidic chip Liver Tissueep
G2, M10
Engineering an in vitro air-blood barrier by 3D bioprinting Lung Tissue
UVEC, A549
Laser direct writing of combinatorial libraries of idealized cellular constructs: Biomedical applications Tissue Engineering
Fibroblast, myoblast, neural stem cells, cancer cells
Bio-printing cell-laden Matrigel agarose constructs Tissue Engineering CT116
PLGA/hydrogel biopapers as a stackable substrate for printing HUVEC networks via BioLP Vascular
UVEC
Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues Vascular
UVEC
Laser assisted bioprinting of engineered tissue with high cell density and microscale organization Tissue Engineering
Rabbit carcinoma cell line B16, HUVEC
The bioink: A comprehensive review on bioprintable materials Tissue Engineering
Review article: several cell types
A review of trends and limitations in hydrogel-rapid prototyping for tissue engineering Tissue Engineering
Review article: several cell types
Three-dimensional printing of biological matters Tissue Engineering
Review article: several cell types
3D scaffolds in breast cancer research Breast Cancer
Review article: several cell types