Are there any special media requirements for organoid culture or is it sufficient to just address the specific needs of that cell type?
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Going tiny is the next BIG thing: Tools and Techniques for Organoid Cultures
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It depends on the organoid of interest as described by a recent commentary by Sato and Clevers. Researchers have been developing and optimizing their favorite organoid medium for kidneys (Xia Y. et al. (2014) Nature Protocols. 9:2693) (Freedman B. et al. Nat Commun. 2015; 6: 8715), brain (Lancaster M.A. and J. A. Knoblich (2014) Nat. Protocols. 9:2329) or lung organoids (Dye B.R. et al. (2015) eLIFE. 4:e05098) (Nikolick and Rawlins, Curr Pathobiol Rep. 2017; 5(2): 223–231). Usually, a good starting point would be the media used for 2D monolayer differentiation with modifications and optimizations.
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