What do you think is the most successful ECM, culture vessel combination for culturing colon cancer spheroids?

Answer

A great option for this type of work would be ultra-low attachment plates like the Corning® spheroid microplate as an optimal cell culture vessel. They are easy to use, promote formation of one organoid per well of uniform size and allow for fluorescent, luminescent and imaging assays to be conducted right in the culture plate. As far as ECMs, Corning Matrigel® matrix has always been considered the gold standard. It is the most cited ECM and is routinely used for 3D cell culture applications. Download the Ultimate Guide to Matrigel matrix or visit Corning’s website to learn more.

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