What is the seeding density that is recommended for using Lipofectamine 3000 to produce lentivirus?

Question

Accepted Answer

Unlike other commonly used transfection reagents that are used in Lentivirus production, Lipofectamine 3000 actually requires a high cell density (cell type: 293T or FT) at the time of transfection.  The recommended density should be 95-99% at the time of transfection.

Answer

I need to package a large gene, which is around a 4-5Kb lentivirus. I used PEI as the transfection reagent, but it gave me very low or almost no lentiviral particles. I was interesting in trying Lipofectamine 3000, do you have any suggestions?

Is it comparable to package lentiviral particles using the forward and reverse transfection methods?

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