A guest blog by Steve Pettit, Director Cell Culture, InVitria with tables provided by The Dish
Dr. Gerhard Gstraunthaler recently presented a seminar with the above title. Gerhard carries a faculty appointment with the Department of Physiology at Innsbruck Medical University, Austria. Gerhard is an advocate of serum-free cell culture technology and is one of the founders of Good Cell Culture Practice (GCCP), an initiative for the standardization of quality control of in vitro studies. (www.GoodCellCulture.com)
Gerhard presented several informative concepts about cell culture and the advantage of using serum-free media. One of the greatest concerns over the use of serum in cell culture media is the variability of results in cell based assays for activity of a growth factor, drug, or other compound on cell function. Serum is undefined and not suitable for most cell based activity assays. The multitude of components found in serum can inhibit the activity of compounds or protein factors in cell based assays. Moreover, serum is variable in composition and variable in performance from on a lot-to-lot basis. Other disadvantages for the use of serum are endotoxin and hemoglobin effects, infectious agents concerns, the lack of performance of FBS with some cell lines, ethical concerns, and the supply concerns over the availability of serum for research or manufacturing purposes.
For consistent cell based performance, serum free media is the best alternative. Serum-free media allow a more controlled culture environment that results in greater reproducibility. There is also less risk of advantageous contamination when animal-free and serum-free media are utilized. Additionally,there is a purification benefit when protein expression is the goal since serum free media have much less protein, including IgG, than serum-supplemented media.
Elimination of the use of serum in cell culture also supports the 3R’s (Refinement, Reduction, Replacement) for the ethical concern of animals.
Gerhard touted the benefits of using Insulin and Transferrin supplements for the reduction of serum in cell culture. An animal free Insulin and Transferrin supplement is available here. Additional reduction of serum can be achieved by adding recombinant albumin such as Cellastim in combination with the Insulin and transferrin supplements.
Gerhard also detailed research, which investigated the use of human platelet extracts as a replacement for serum. These extracts serve as an equivalent replacement for serum in many cell types such as stem cells and epithelial cells.
|Type||Product||Cost Per Liter of Media||Animal-free, Animal Derived||Recombinant or Native Components|
|Insulin-Transferrin Supplement||InVitria ITSE Animal-free||$10-12||Animal Free||Recombinant|
|Fisher Sci AF ITSE||$12-$14||Animal Free||Recombinant|
|Corning/Mediatech ITS||$15-20||Animal Derived||Native|
|Invitrogen ITS-A||$20-25||Animal Derived||Native|
|Invitrogen ITS-G||$20-25||Animal Derived||Native|
|Invitrogen ITS-X||$20-$25||Animal Derived||Native|
|Gemini BIO ITS||$20-25||Animal Derived||Native|
|Sigma ITS||$12-15||Animal Derived||Native|
|ThermoFisher ITS||$12-15||Animal Derived||Native|
|BD ITS +||$12-15||Animal Derived||Native|
|Sciencell ITS||$15-20||Animal Derived||Native|
|Recombinant Albumin||InVitria Cellastim||$1-6||Animal Free||Recombinant/td>|
|Sigma rAlbumin||$1-6||Animal Free||Recombinant|
|Fisher Sci rAlbumin||$1-6||Animal Free||Recombinant|
|Mediatech cellgro rAlbumin||$1-6||Animal Free||Recombinant|
|Sheffield Bioscience Albumin ACF||$1-6||Animal Free||Recombinant|