Are differences in cell viability levels from one transfection method to another large enough to actually affect protein titers?
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Maximizing Transient Protein Production
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Yes, high cell viability is critical for achieving high titers. Of course, it is essential to use cells that exhibit high viability pre-transfection (>95%), but it should also be emphasized that if cell viability is impacted by the transfection process, then cells will also exhibit reduced productivity. We consistently observed that post transfection viability >90% leads to titers that are significantly higher compared to cells that show viability <90% post transfection.
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