Do you feel antibiotics are necessary in the culture, I would prefer not to use them.
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Using Intestinal Organoids for Intestinal and Stem Cell Research
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Antibiotics are only necessary as a preventative measure in the culture. The medium is not affected positively or negatively by their addition. Intestinal culture is inherently a very non sterile tissue and that is why we recommend the addition of antibiotics. But they can be cultured without antibiotics if you prefer not to use them.
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