Do you have a protocol for predicting differentiation or is this something that needs to be developed by the lab using it?
This question is part of the following Ask The Expert session:
Using Luminex Technology to Non-Invasively Monitor Stem Cell Differentiation
Answered by:
Company: Bio-Techne
Job Title: Manager of Product Development for the Stem Cell and Cell Culture Department
Answer
We do not currently have a protocol for predicting successful differentiation. This in part due to our observation that the profile of successful differentiation is dependent on the downstream differentiated cell of interest. For example, we find that hepatocytes and cardiomyocytes have unique secretory profiles when differentiated from iPSCs. We suggest that each lab independently determine the secretory profile for their differentiated cell type of interest. This can be done qualitatively using the Proteome Profiler Arrays, followed by the more quantitative Luminex Assays.
This post is sponsored by Bio-Techne.