Do you have any recommendations on a coating matrix that you use for MSCs?


If you are using serum-containing medium, a tissue culture-treated flask should work just fine without the need for any additional treatment for good MSC growth. However, once you withdraw serum, MSC adhesion can become an issue. 1-5 ug/squared centimeter of human serum fibronectin is probably the simplest way to recover MSC adhesion in the absence of serum. There are also a number of xeno-free defined commercially available matrices such as CellStart that also work very well. In our lab, we actually prefer not to coat flasks as this is a bit time consuming and can get expensive. We use the CellBIND surface from Corning and have good luck with cell adhesion and maintenance of multipotency in MSC.