Do you have any recommendations on a coating matrix that you use for MSCs?


If you are using serum-containing medium, a tissue culture-treated flask should work just fine without the need for any additional treatment for good MSC growth. However, once you withdraw serum, MSC adhesion can become an issue. 1-5 ug/squared centimeter of human serum fibronectin is probably the simplest way to recover MSC adhesion in the absence of serum. There are also a number of xeno-free defined commercially available matrices such as CellStart that also work very well. In our lab, we actually prefer not to coat flasks as this is a bit time consuming and can get expensive. We use the CellBIND surface from Corning and have good luck with cell adhesion and maintenance of multipotency in MSC.

Pin It on Pinterest