This question is part of the following Ask The Expert session:
The primary culture of these cells is sometimes finicky as many variables regarding the mice affect the cells, and we have optimized IntestiCult™ Organoid Growth Medium (Mouse) to account for this. As for easy or challenging that can depend on your definition. On one hand culturing organoids is easy as you can be confident that you will generate organoids during your first attempt when following our protocol. On the other hand, it is challenging enough to keep your interest, especially with the wide variety of applications that are possible with these cultures.