I am working on reducing serum in our iPCS culture. What steps would you recommend for weaning and in your experience how low have you gotten in serum concentration?
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I would highly recommend a step wise reduction in serum for iPSC over multiple passages. There are a variety of serum free medias currently marketed for iPSCs, so these cells can be propagated in serum free conditions. In addition, we have developed in house animal component free formulations that are capable of expanding iPSC for at least 12 passages while retaining all of the desired phenotypic traits. When combined with a recombinant vitronectin matrix, these cells can be expanded in media that is completely void of any human or animal serum components. If you are interested in formulations such as these, we can further discuss
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