I am working to create a model system for liver diseases and toxicity testing. Do you have a StemDiff protocol for other disease model systems or just neurological disease?

Answer

For differentiation of ES and iPS cells to hepatocytes we recommend using the STEMdiff™ Definitive Endoderm Kit to generate definitive endoderm, in conjunction with Dr. David Hay’s protocol for downstream differentiation (Hay et al. Stem Cells, 2008). Cells differentiated using the STEMdiff™ Definitive Endoderm Kit express high levels of endoderm markers, including CD184 (CXCR4), SOX17, FOXA2 and c-Kit, and lack expression of ectoderm, mesoderm and pluripotency markers. The definitive endoderm produced using this kit is multipotent and capable of further differentiation towards the hepatic lineage (and pancreatic and pulmonary, for that matter). If you’re interested to hear more about how this kit has been used by Dr. David Hay to generate metabolically active hepatocytes, please view this complimentary view-on-demand webinar:

http://www.stemcell.com/en/Forms/Multimedia/Archived-Webinar-Derivation-of-Metabolically-Active-Hepatocytes-from-Human-Pluripotent-Stem-Cells.aspx