In reprogramming cord blood cells, we have found that our cells are detaching, thus negatively affecting our reprogramming efficiency. Any suggestions?
This question is part of the following Ask The Expert session:
Using Blood Cells As a Source for Generating Induced Pluripotent Stem Cells
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It is difficult to say what may be causing detachment of cells and whether the cells that are detaching are blood cells that are not being reprogrammed and are dying over long-term culture. If the cells that are detaching are pre-iPS cell-like colonies, I would re-examine the vector system and matrix, and whether the medium you are using is optimal for blood cells. We developed ReproTeSR™ specifically for reprogramming of blood-derived cells, and would therefore recommend that medium for your situation.
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