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Both the detailed procedure and IntestiCult™ Organoid Growth Medium (Mouse) have been designed with that thought in mind. The step-by-step manual is easy to follow and the robustness of the medium allows for a bit of a “learning curve”. Although mastering any new skill takes some practice, the time to get “up and running” with organoid cultures should be very quick, and usually achievable during the first attempt. Good luck!