Do you include any other proteins or growth factors in your media besides the albumin and transferrin?
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Yes. Most cell types require supplementation with cytokines. There are several different sources of cytokines that are considered to be animal free and produced in E. coli. The choice of which cytokines that should be used will be dependent on the cell type that is trying to be expanded. For example, T cells require IL-2 where as VERO expansion is dependent on EGF.
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