Is it always important to supplement media with depleted components?
This question is part of the following Ask The Expert session:
Troubleshooting Cell Culture Media for Bioprocessing
Company: Cytiva (formerly GE)
Job Title: Director of Process Science
Nutrient depletion causes cell apoptosis which can impact productivity levels and/or product quality therefore it is important to supplement media with depleted components. In order to achieve the best performance, a metabolically balanced feed formulation and an optimal feeding schedule should be designed and applied.