Is there a reason that you are using limited dilution rather than some of the imaging systems to prove clonality?

Answer

Limited dilution to provide high probability of monoclonality or limited dilution with an image to provide supporting evidence of monoclonality are adequate ways of assurance that the cell lines have a high probability of being clonally derived.  We have the capability to use both limited dilution and integrated, automated imaging to support clonality.

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