It’s become more and more difficult to get glass pipettes. Do you use glass pipettes or plastic pipettes for this kit of differentiation and for cell culture without antibiotics ?

This question is part of the following Ask The Expert session:

Differentiating Human ES and iPS Cells to Pancreatic Progenitor Cells

Answered by:

Dr. Michael Riedel

Company: STEMCELL Technologies Inc.

Job Title: Senior Scientist

Answer

The media comprising the STEMdiff™ Pancreatic Progenitor Kit are compatible with standard plastic micropipette tips. We recommend that the user practice sterile technique and avoid the use of antibiotics.

Previous Question:

We are trying this kit to differentiate human pancreatic precursors from H1 cells. We found that from Stage 3 the cells became multilayers. We stained stage 4 cells on cell culture plate, but because the cells clusters are thick, we can’t get clear images using common immunofluorescence microscope . How did you prepare samples for the Stage 4 immunohistochemistry assay? Did you culture the cells on cell culture plate, or on the coverslips? Did you get the images from Confocal Microscope?

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