How many rounds of secretion profiles did it take you to start to see a differentiation pattern?
This question is part of the following Ask The Expert session:
Using Luminex Technology to Non-Invasively Monitor Stem Cell Differentiation
Answered by:
Company: Bio-Techne
Job Title: Manager of Product Development for the Stem Cell and Cell Culture Department
Answer
We are still undergoing this process to find signatures that will correlate with differentiation success. We have done up to 3 replicates at this point and are focusing on molecules that were seen both in the Array and confirmed via Luminex. We found very consistent results. It will be important to then test these analytes across more cell lines as well as different protocols to confirm our findings.
This post is sponsored by Bio-Techne.