Have you produced viral vectors using CHO? Have you seen any differences or benefits over using HEK?
This question is part of the following Ask The Expert session:
Maximizing Transient Protein Production
Job Title: Director of Cell Culture
In general, we find our clients are using HEK, Vero, or insect cells for producing viral vectors or VLPs. Thus, our research scientists do not have direct experience transfecting CHO cells specifically for viral vector production. We certainly have produced a variety of vaccines and viral vectors in other commonly used cell lines.