Would you recommend a media change post transfection or could you tell me how to avoid having to change the media. How long to culture post transfection?

Answer

After transfection, we typically do not change media, unless we are performing selection for stable pool or clone generation. The high level of cell viability following MaxCyte electroporation allows not only for rapid protein production, but also for longer-term culturing to maximize total protein yield. Thus, the length of post transfection culture depends on the amount of protein that is needed. Some customers prefer to harvest after only 5-7 days of culture. However, using fed-batch cultures, we have seen transient titers steadily increase for up to 3 weeks post transfection.