This question is part of the following Ask The Expert session:
After transfection, we typically do not change media, unless we are performing selection for stable pool or clone generation. The high level of cell viability following MaxCyte electroporation allows not only for rapid protein production, but also for longer-term culturing to maximize total protein yield. Thus, the length of post transfection culture depends on the amount of protein that is needed. Some customers prefer to harvest after only 5-7 days of culture. However, using fed-batch cultures, we have seen transient titers steadily increase for up to 3 weeks post transfection.