Have you seen any effects of serum in the media or CD media having an impact on your ability to study the secretion profiles?
This question is part of the following Ask The Expert session:
Using Luminex Technology to Non-Invasively Monitor Stem Cell Differentiation
Answered by:
Company: Bio-Techne
Job Title: Manager of Product Development for the Stem Cell and Cell Culture Department
Answer
You will definitely detect various growth factors/cytokines from the media you are using, especially if you are culturing your cells with growth factors/cytokines. We would recommend running samples of media alone as a control to ensure that the proteins you are detecting through the arrays and/or Luminex are due to secretion by cells and not from the media.
This post is sponsored by Bio-Techne.