Have you seen any effects of serum in the media or CD media having an impact on your ability to study the secretion profiles?


You will definitely detect various growth factors/cytokines from the media you are using, especially if you are culturing your cells with growth factors/cytokines.  We would recommend running samples of media alone as a control to ensure that the proteins you are detecting through the arrays and/or Luminex are due to secretion by cells and not from the media.

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