This might be a little off topic but how are you proving monoclonality? Is it part of your automated process?
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Adapting manual cell line development workflows to automation
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Company: Alexion Pharmaceuticals, Inc.
Job Title: Development Associate IV, Biological Process Development
Answer
Monoclonality is established using probability based on limited dilution. We also have the ability to image plates for assurance of monoclonality using a plate imager that is integrated into the automated platform.