How has the transition from xeno-free or FBS supplemented media to your formulations been? Is it a difficult transition for the cells?

Question

Accepted Answer

Typically transition from xeno-free or FBS -supplemented media is straight forward. With direct adaptation, cells usually adapt within a passage and do well in the blood-free media in subsequent passages. However, adaptation is cell-type specific and some cell types require a step-wise gradual transition from xeno-free media to blood-free.

Answer

For which primary cell lines have you been able to create a blood-free media? Do you find that different cell lines require varying levels of albumin/transferrin?

Are these supplements or are these formulated into the media. Do your proteins need to be added during culture or just in the beginning?

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