How has the transition from xeno-free or FBS supplemented media to your formulations been? Is it a difficult transition for the cells?
This question is part of the following Ask The Expert session:
Incorporating recombinant proteins to produce blood-free media formulations for therapeutic cell types
Job Title: Cell Culture Scientist
Typically transition from xeno-free or FBS -supplemented media is straight forward. With direct adaptation, cells usually adapt within a passage and do well in the blood-free media in subsequent passages. However, adaptation is cell-type specific and some cell types require a step-wise gradual transition from xeno-free media to blood-free.