We are running perfusion culture so our runs are over a month long. Can the inline sampling be adapted for that kind of timeframe?

Answer

“At-line testing” using the Cedex analyzers requires you to obtain a small amount of sample (minimum of about 100 µl) from the culturing system for testing. The big advantage in comparison to an integrated in-line system is that an alteration or failure of an internal sensor in the bioreactor would be fatal because process control is no longer possible (severe process risk). On the other hand, the at-line instrument can be easily maintained and you can rely on continuous process control with high data quality, and the instrument has the flexibility to be placed in close vicinity to the culture or in a dedicated lab, whatever fits better in the individual environment.