Which serum free media is suitable for optimization of CHO and BHK cells (Cell Culture) producing recombinant blood clotting factors.
This question is part of the following Ask The Expert session:
Troubleshooting Cell Culture Media for Bioprocessing
Company: Cytiva (formerly GE)
Job Title: Director of Process Science
There are commercially available serum free media suitable for the optimization of recombinant blood factor expression in CHO or BHK cells. Depending on the cell type and specific molecule of interest, the media may need to be supplemented with other components (e.g., growth factors, cell protection agents, and product dependent factors). Media optimization should always be performed in conjunction with process optimization (e.g. fed-batch vs. perfusion, tuning of process parameters) to achieve optimal outcomes.