This video outlines the steps in semi-solid cloning of mouse hybridomas to generate mouse monoclonal antibodies using ClonaCell™-HY. The cloning protocol demonstrated in this video can also be used to clone other mammalian cell types with other ClonaCell™ semi-solid media products. Steps covered in this video include preparation of myeloma cells and splenocytes, fusion, selection and cloning, and harvesting

[youtube id=”u-kueNZPrlU” width=”640″ height=”360″]