Jennifer Antonchuck, Michael J. Riedel, Mark Fairey, Simon Hilcove, Andrew Elefanty, Elizabeth Ng, Terry E. Thomas, Allen C. Eaves and Sharon A. Louis
Human pluripotent stem cells (hPSCs), including embryonic stem cells (hESC) and induced pluripotent stem cells (hiPSC), are characterized by their potential to differentiate into any somatic cell lineage. A diverse set of multi-step directed differentiation protocols have been described to induce differentiation to desired cell types, using a variety of media formulations, in embryoid body (EB)-based or adherent cell-based cultures. We have developed STEMdiff™ APEL™ Medium, a fully defined, animal component-free medium based on the publication of Ng et al., which can be used as a base medium for differentiation of hPSCs to multiple lineages, in EB or adherent cell platforms. Here we demonstrate the utility of STEMdiff™ APEL™ Medium as a base media for cardiomyocyte, definitive endoderm or hematopoietic differentiation using cytokine combinations from the literature.