How efficient is cell attachment? Are modifications of the attachment procedure necessary?
This question is part of the following Ask The Expert session:
Enabling Stem Cell Therapy Biomanufacturing using Dissolvable Microcarriers
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Answer
Cell attachment to Corning Dissolvable Microcarriers is quite efficient: We typically see greater than 90% attachment as determined by the count of free cells in the supernatant. There is a significant range of time required to attach for various processes, however. The characteristics of cell type and/or media used can impact how readily attachment occurs. In examples of cells cultivated in 5L bioreactors, cell attachment can occur within 4 hours given continuous agitation or may require overnight attachment with an intermittent agitation regimen. As seen with other microcarriers, some cells attach better when processing occurs at ½ of the final working volume, low or no serum present at attachment, and with differing agitation conditions.
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