I am having trouble retaining differentiation potential after thawing, any recommendations? Thank you!
This question is part of the following Ask The Expert session:
Directed Differentiation of Pluripotent Stem Cells
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Company: Thermo Fisher Scientific, Inc.
Job Title: Director of Research and Development
Answer
Generally it depends the efficiency of your differentiation method. But if you are seeing the problem due to freezing and thawing, try ThermoFisher’s RevitaCell™ Supplement (100X), on the day the cells are thawed. Following 24hrs in culture, there is no need to use this supplement. Detailed instructions are available. Also some limitations apply, as some specific cells may not respond. http://www.lifetechnologies.com/order/catalog/product/A2644501?ICID=search-product
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