Are the ALI and sphere culture methods compatible with hPSC-derived airway cells?
This question is part of the following Ask The Expert session:
Culturing epithelial cells using advanced 3D culture systems for airway modeling
Company: STEMCELL Technologies Inc.
Job Title: Senior Scientist
Yes. We have several customers who have been using PneumaCult media for expansion and maturation of their hPSC-derived lung progenitors using sphere and ALI culture methods. Most interestingly, a recent publication by Konishi et al. demonstrated that combination of a 3D sphere culture methodology with PneumaCult-ALI medium promotes differentiation of hPSC-derived lung progenitors to proximal multi-ciliated airway cells.