Are the ALI and sphere culture methods compatible with hPSC-derived airway cells?


Yes. We have several customers who have been using PneumaCult media for expansion and maturation of their hPSC-derived lung progenitors using sphere and ALI culture methods. Most interestingly, a recent publication by  Konishi et al. demonstrated that combination of a 3D sphere culture methodology with PneumaCult-ALI medium promotes differentiation of hPSC-derived lung progenitors to proximal multi-ciliated airway cells.

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