What is the typical or expected % cilia in an ALI airway culture?
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Culturing epithelial cells using advanced 3D culture systems for airway modeling
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The ciliated cells can make up ~80% of the total apical cells in the in vivo human airway. In our fully differentiated ALI cultures using PneumaCult media, the percentage of cultures that are composed of ciliated cells ranges from 80% for cultures initiated with passage 2 cells to 30% for cultures initiated with passage 4 cells