Have you or anyone you know automated this process for high throughput screens?
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Culturing epithelial cells using advanced 3D culture systems for airway modeling
Company: STEMCELL Technologies Inc.
Job Title: Senior Scientist
We haven’t performed high-throughput assays in-house at this time. Regarding the sphere culture system, Danahay et al. used a 384-well format for bronchospheroid culture as the basis of a high-throughput screening system. Regarding the ALI culture system, there are 96-well format cell culture inserts that are commercially available, but not an off-the-shelf automated system. There is, however, an automated ALI culture system that uses PneumaCult medium published by Aufderheide et al.