Can you use your system for culturing diseased ALI cells as well?
This question is part of the following Ask The Expert session:
Culturing epithelial cells using advanced 3D culture systems for airway modeling
Company: STEMCELL Technologies Inc.
Job Title: Senior Scientist
Yes, the PneumaCult culture system can be used for diseased cells as well as cells from healthy donors. Our collaborators and customers have used PneumaCult media for nasal epithelial cells isolated from asthmatic patients (Reeves et al., 2015; Xu et al., 2015) and airway epithelial cells from cystic fibrosis patients (Reeves et al., 2014) Air-liquid-interface and sphere cultures of human airway cells provide excellent model systems to interrogate the cellular characteristics inherent in these and other respiratory disease states in vitro.