Are sphere cultures of airway epithelial cells compatible with ICC analysis? What other downstream assays and analysis methods work well for this culture system?

Answer

Yes, airway epithelial cells grown in sphere cultures are compatible with ICC analysis. Contact our Product and Scientific Support department to request the protocol for isolating spheres from MatrigelĀ® (techsupport@stemcell.com). Regarding downstream assays, there are many possibilities, depending on the specific research questions you are using the sphere cultures to address. For example, the spheres from each well can be isolated and assessed for the presence of specific markers by subjecting cells to either qPCR analysis, to quantify the level of specific RNA transcripts present in the cells, or western blot analysis, to assess the expression levels of specific proteins.