What type of cells can be used to initiate ALI cultures and how long can the ALI cultures be maintained?

Answer

Commercially available human airway epithelial cells are compatible with air-liquid interface (ALI) culture in PneumaCult-Ex™ and PneumaCult-ALI™ when they are seeded onto cell culture inserts no later than Passage 4. Using cells at later passages will result in a significant decrease in quality or failure of ALI culture. PneumaCult media also work well with primary human nasal and bronchial epithelial biopsies. In addition to human airway epithelial cells, PneumaCult media also work well with ferret and mouse airway epithelial cells.

ALI cultures can be maintained up to a year if there are no issues with culture contamination.