Are there any special considerations for using the FluoroBrite media? Additional media changes, etc.
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Improving Live Cell Fluorescence Imaging
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FluoroBrite media works just like regular, CO2-buffered DMEM media. Just add the same concentration of FBS and/or any other supplement that you would normally add to regular DMEM and image your cells in a 5% CO2, 37°C and humidified environment.