Are there media components to avoid that could interfere with imaging?
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The main media components that interfere with imaging are phenol red and vitamins. Phenol red quenches fluorescence in both the green and red emission channels causing a substantial reduction in the signal-to-noise ratio of a fluorophor. Vitamins autofluoresce particularly in the green channel and this effect will also reduce the signal-to-noise ratio of a fluorophore.
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