Are there media components to avoid that could interfere with imaging?

Answer

The main media components that interfere with imaging are phenol red and vitamins. Phenol red quenches fluorescence in both the green and red emission channels causing a substantial reduction in the signal-to-noise ratio of a fluorophor. Vitamins autofluoresce particularly in the green channel and this effect will also reduce the signal-to-noise ratio of a fluorophore.